Alla Kostyukova, PhD

Biographical Information

Alla Kostyukova received her M.Sc. in Biophysics from St. Petersburg State University, Russia, and Ph.D. in Molecular Biology from the Institute of Protein Research, Russian Academy of Sciences & Moscow State University. She worked as a Research Associate in the Institute of Protein Research, Russia, and as a Senior Research Associate in RIKEN Harima Institute at SPring8, Japan, before moving to the USA. Before joining the Washington State University Voiland School faculty she worked for 4 years as an Assistant Professor and Director of the Circular Dichroism Facility in Robert Wood Johnson Medical School, Piscataway, NJ.

Research Interests

1. Regulation of actin dynamics in cytoskeleton of muscle and non-muscle cells. Playing a critical role in muscle contraction, cell movement, and cell morphology, actin filaments form vastly different structures in different cell types and different locations within the cell. Regulation of the dynamics at actin’s ends is of central importance in the assembly of these various structures. The major goal of our research is to determine the mechanisms of this fine-tuned regulation necessary to understand processes existent in living cells, such as myocytes and neurons.
2. Protein structure, protein-protein interactions and protein engineering. Important part of our research is studying structure of proteins and formation of protein complexes as well as engineering proteins with desired properties. These studies are parts in our ongoing projects as well as incollaborative projects.

Ongoing Research Projects

• NIH RO1 GM120137, 06/01/2017-05/31/2021
  Regulation of the actin filament pointed end dynamics in health and disease.

List of Recent Publications

(full list of publications: https://goo.gl/m5ixoB )

1. Ly T, Pappas CT, Johnson D, Schlecht W, Colpan M, Galkin VE, Gregorio CC, Dong WJ, Kostyukova AS. Effects of cardiomyopathy-linked mutations k15n and r21h in tropomyosin on thin filament regulation and pointed-end dynamics. Mol Biol Cell. 2019; 30(2):268-281. DOI: 10.1091/mbc.E18-06-0406.
2. Gray KT, Stefen H, Ly TNA, Keller CJ, Colpan M, Wayman GA, Pate E, Fath T, Kostyukova AS. ETropomodulin’s Actin-Binding Abilities Are Required to Modulate Dendrite Development. Front Mol Neurosci. 2018; 11:357. DOI: 10.3389/fnmol.2018.00357
3. Tolkatchev D, Elnatan D, Nogara L, Ly T, Naber N, Haak K, Meech R, Cooke R, Kostyukova AS. Piperine, an alkaloid inhibiting the super-relaxed state of myosin, binds to the myosin regulatory light chain. Arch Biochem Biophys. 2018; 659:75-84. DOI: 10.1016/j.abb.2018.09.027
4. Arslan B, Colpan M, Gray KT, Abu-Lail NI, Kostyukova AS. “Characterizing interaction forces between actin and proteins of the tropomodulin family reveals the presence of the N-terminal actin-binding site in leiomodin.” Arch Biochem Biophys. 2017. 638:18-26. DOI: 10.1016/j.abb.2017.12.005
5. Ly T, Krieger I, Tolkatchev D, Krone C, Moural T, Samatey FA, Kang C, Kostyukova AS. “Structural destabilization of tropomyosin induced by the cardiomyopathy-linked mutation R21H.” Protein Sci. 201. DOI: 10.1002/pro.3341.
6. Colpan, M., Ly, T., Grover, S., Tolkatchev, D., Kostyukova, A.S. “The cardiomyopathy-associated K15N mutation in tropomyosin alters actin filament pointed end dynamics.” Arch Biochem Biophys. 2017. 630:18-26. DOI: 10.1016/j.abb.2017.07.006.
7. Barker, C.S., Meshcheryakova, I.V., Kostyukova, A.S., Freddolino, P.L., Samatey, F.A. “An intrinsically disordered linker controlling the formation and the stability of the bacterial flagellar hook.” BMC Biol. 2017. 15(1):97. DOI: 10.1186/s12915-017-0438-7.
8. Gray, K.T., Kostyukova, A.S., Fath, T. “Actin regulation by tropomodulin and tropomyosin in neuronal morphogenesis and function.” Mol Cell Neurosci. 2017. pii: S1044-7431(16)30207-X. DOI: 10.1016/j.mcn.2017.04.002.
9. Colpan, M., Ly, T., Grover, S., Tolkatchev, D., Kostyukova, A.S.. “The cardiomyopathy-associated K15N mutation in tropomyosin alters actin filament pointed end dynamics.” Arch Biochem Biophys. 2017. 630:18-26.
10. Ly, T., Moroz, N.A., Pappas, C.T., Novak, S.M., Tolkatchev, D., Wooldridge, D., Mayfield, R.M., Helms, G., Gregorio, C.C., Kostyukova, A.S. “The N-terminal tropomyosin- and actin-binding sites are important for leiomodin 2’s function.” Molecular Biology of the Cell, 2016. 27:16 2565-2575. DOI: 10.1091/mbc.E16-03-0200.
11. Gray, K.T., Suchowerska, A.K., Bland, T., Colpan, M., Wayman, G., Fath, T., Kostyukova, A.S. “Tropomodulin isoforms utilize specific binding functions to modulate dendrite development.” Cytoskeleton, 2016. 73(6):316-28. DOI: 10.1002/cm.21304.
12. Colpan, M., Moroz, N.A., Gray, K.T., Cooper, D.A., Diaz, C.A., Kostyukova, A.S. “Tropomyosin- and actin-binding properties of tropomodulins modulate their competition for binding at the pointed end of actin filaments.” Arch Biochem Biophys. 2016. 600:23-32. DOI: 10.1016/j.abb.2016.04.006.
13. Arslan, B., Colpan, M., Ju, X., Zhang, X., Kostyukova, A.S., Abu-Lail, N. “The effects of noncellulosic compounds on the nanoscale interaction forces measured between carbohydrate-binding module and lignocellulosic biomass.” Biomacromolecules. 2016. 17(5):1705-15. DOI: 10.1021/acs.biomac.6b00129.
14. Colpan, M., Tolkatchev, D., Grover, S., Helms, G.L., Cort, J., Moroz, N.A., Kostyukova, A.S. “Localization of the Binding Interface between Leiomodin-2 and α-Tropomyosin.” Biochim Biophys Acta. 2016. 1864(5):523-30.
15. van Dijk, S.J., Kooiker, K.B., Mazzalupo, S., Yang Y., Kostyukova, A.S., Mustacich, D.J., Hoye, E.R., Stern, J.A., Kittleson, M.D., Harris, S.P. “The A31P missense mutation in cardiac myosin binding protein C alters protein structure but does not cause haploinsufficiency.” Arch Biochem Biophys. 2016. 601:133-140.