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The Gene and Linda Voiland School of Chemical Engineering and Bioengineering

Faculty & Staff

Dmitri Tolkatchev, PhD

Dmitri Tolkatchev

Dmitri Tolkatchev, Ph.D.
Assistant Research Professor

Working with Dr. Alla Kostyukova

Protein chemistry and structure, protein-protein and protein-ligand interactions; protein engineering; protein NMR

The Gene and Linda Voiland
School of Chemical Engineering and Bioengineering
1505 Stadium Way, Room 105
P.O. Box 646515
Washington State University
Pullman, WA 99164-6515

Biography

Dmitri graduated with honors in 1982 after 6 years of studies at the Department of Physicochemical Biology, Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia. He then switched to research in organometallic chemistry and in 1992 he received a Ph.D. in Chemistry from the A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences. He joined the Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, in 1993 as a postdoc where he studied molecular mechanisms of the mitochondrial electron transport chain. In 1996 he moved to Montreal, Canada and continued his work first at McGill University, and later in the Biomolecular NMR lab of the Biotechnology Research Institute, at the National Research Council Canada. In 2014 he joined WSU as a Research Assistant Professor.

Research interests

  • Protein and peptide engineering
  • Biomolecular interactions
  • Structure and function of proteins involved in cytoskeleton formation
  • Folding and biological function of growth factors from the granulin family

Other interests

  • Machine learning
  • Statistics

Ongoing Research Projects

NIH RO1 GM120137, 06/01/2017-05/31/2021
Regulation of the actin filament pointed end dynamics in health and disease.
Role: Co-Investigator

Recent publications:

  1. Tolkatchev D, Elnatan D, Nogara L, Ly T, Naber N, Haak K, Meech R, Cooke R, Kostyukova AS. Piperine, an alkaloid inhibiting the super-relaxed state of myosin, binds to the myosin regulatory light chain. Arch Biochem Biophys. 2018 Dec 1;659:75-84.
  2. Tolkatchev D. Nuclear Magnetic Resonance Spectroscopy in Analysis of Granulin Three-Dimensional Structure and Cysteine Bridging. Methods Mol Biol. 2018;1806:65-80.
  3. Tolkatchev D. Large-Scale Generation of Recombinant Granulin Peptides in E. coli. Methods Mol Biol. 2018;1806:51-64.
  4. Ly T, Krieger I, Tolkatchev D, Krone C, Moural T, Samatey FA, Kang C, Kostyukova AS. Structural destabilization of tropomyosin induced by the cardiomyopathy-linked mutation R21H. Protein Sci. 2018 Feb;27(2):498-508.
  5. Colpan M, Ly T, Grover S, Tolkatchev D, Kostyukova AS. The cardiomyopathy-associated K15N mutation in tropomyosin alters actin filament pointed end dynamics. Arch Biochem Biophys. 2017 Sep 15;630:18-26.
  6. Ly T, Moroz N, Pappas CT, Novak SM, Tolkatchev D, Wooldridge D, Mayfield RM, Helms G, Gregorio CC, Kostyukova AS. The N-terminal tropomyosin- and actin-binding sites are important for leiomodin 2’s function. Mol Biol Cell. 2016 Aug 15;27(16):2565-75.
  7. Colpan M, Tolkatchev D, Grover S, Helms GL, Cort JR, Moroz N, Kostyukova AS. Localization of the binding interface between leiomodin-2 and α-tropomyosin. Biochim Biophys Acta. 2016 May;1864(5):523-30.

Patents:

  1. Ni,F., Xu,P., Hossain,S., Tolkatchev,D. Derivatives of collagen-binding hairpin peptides, International patent WO2014059530, 2014-04-24
  2. Ni,F., Ng,A., Wang,P., Tolkatchev,D. Multimerized single domain antibody ligands of prothrombin as inhibitors of blood and extravascular coagulation, International patent WO 2013123591, 2013-08-29
  3. Ni,F., Tolkatchev,D., Tonan, K., Xu, P., Hossain, S., Locally active anticoagulants and antiflammatory agents, International patent WO2012142696, 2012-10-26; also published as US20140113854, CA2833614A1
  4. Filfil,R., Tolkatchev,D., Ni,F., O’Connor-McCourt,M.D., Lenferink, A.E.G., Peptide ligands for clusterin and uses thereof, International patent WO2010118521, 2010- 10-21; also published as US20120121507, CA2756570A1, EP2419440A1, EP2419440A4
  5. Ni,F., Tolkatchev,D., Su,Z., Polypeptide ligands containing linkers, International patent WO2006000081, 2006-01-05; also published as US20090105116, US8062018, CA2571815A1, EP1766011A1, EP1766011A4, EP1766011B1
  6. Ni,F., Su,Z., Tolkatchev,D., Natapova,A., Koutychenko,A. Peptide inhibitors of thrombin as potent anticoagulants, International patent WO2004076484, 2004-09- 10; also published as US7456152, US20070042946, US20090137779, CA2515798A1, EP1597279A1
  7. Ni,F., Su,Z., Xu,P., Tolkatchev,D., Osborne,M., Koutychenko,A. Quantitative ranking of transient ligand binding to target biomolecules by use of nuclear magnetic resonance, International patent WO2003057258, 2003-07-17; also published as US20050287527, CA2472193A1, EP1463952A2
Alla Kostyukova's team: Kyle Swain, Garry Smith, Thu Ly, Dr. Dmitri Tolkatchev, and Tanzila Islam
Alla Kostyukova’s research team (left to right): Kyle Swain, Garry Smith, Thu Ly, Dr. Dmitri Tolkatchev, and Tanzila Islam